Angiotensin II ELISA Kit

Enzyme-linked Immunosorbent assay-Competitive

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Angiotensin II is an octapeptide that is a potent but labile vasoconstrictor. It is produced from angiotensin I after the removal of two amino acids at the C-terminal by Angiotensin converting enzyme. The amino acid in position 5 varies in different species. To block vasoconstriction and hypertension effect of angiotensin II, patients are often treated with ace inhibitors or with Angiotensin II Type I receptor blockers.

Assay Methodology: Quantitative Competitive ELISA

Aviva Systems Biology Angiotensin II ELISA Kit (OKEH02526) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Angiotensin II antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Angiotensin II and incubated. The Angiotensin II found in the sample or standards competes with the biotinylated Angiotensin II for limited binding sites on the immobilized anti-Angiotensin II antibody. Excess unbound biotinylated Angiotensin II and sample or standard Angiotensin II is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Angiotensin II captured in the well and inversely proportional to the amount of Angiotensin II which was contained in the sample or standard.

Mean Intra-Assay Precision: %CV < 5.5%, n=20
Mean Inter-Assay Precision: %CV < 8.1%, n=20%