Hyaluronic acid ELISA Kit

ELISA Kit

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Store as indicated in product manual.

Hyaluronic acid

Assay Methodology: Quantitative Competitive ELISA

Aviva Systems Biology Hyaluronic acid ELISA Kit (OKEH02527) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Hyaluronic acid antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Hyaluronic acid and incubated. The Hyaluronic acid found in the sample or standards competes with the biotinylated Hyaluronic acid for limited binding sites on the immobilized anti-Hyaluronic acid antibody. Excess unbound biotinylated Hyaluronic acid and sample or standard Hyaluronic acid is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Hyaluronic acid captured in the well and inversely proportional to the amount of Hyaluronic acid which was contained in the sample or standard.

Mean Intra-assay CV%: < 6.4% (n = 20)
Mean Inter-assay CV%: < 9.2% (n = 20)