Nitrotyrosine ELISA Kit

ELISA Kit

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Nitrotyrosine

Assay Methodology: Quantitative Competitive ELISA

Aviva Systems Biology Nitrotyrosine ELISA Kit (OKEH02600) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Nitrotyrosine antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Nitrotyrosine and incubated. The Nitrotyrosine found in the sample or standards competes with the biotinylated Nitrotyrosine for limited binding sites on the immobilized anti-Nitrotyrosine antibody. Excess unbound biotinylated Nitrotyrosine and sample or standard Nitrotyrosine is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Nitrotyrosine captured in the well and inversely proportional to the amount of Nitrotyrosine which was contained in the sample or standard.

Mean Intra-assay CV%: < 4.5% (n = 20)
Mean Inter-assay CV%: < 6.6% (n = 20)