Prostaglandin D2 ELISA Kit

ELISA Kit

Root Catalog/Products/ELISA Kits, Root Catalog/Products/ELISA Kits/Biochemicals Hormones

Store as indicated in product manual.

Prostaglandin D2

Assay Methodology: Quantitative Competitive ELISA

Aviva Systems Biology Prostaglandin D2 ELISA Kit (OKEH02602) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Prostaglandin D2 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Prostaglandin D2 and incubated. The Prostaglandin D2 found in the sample or standards competes with the biotinylated Prostaglandin D2 for limited binding sites on the immobilized anti-Prostaglandin D2 antibody. Excess unbound biotinylated Prostaglandin D2 and sample or standard Prostaglandin D2 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Prostaglandin D2 captured in the well and inversely proportional to the amount of Prostaglandin D2 which was contained in the sample or standard.

Serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids