Angiotensin I ELISA Kit

Enzyme-linked Immunosorbent assay-Competitive

Wet Ice

Angiotensins are small peptides derived from angiotensinogen. Several of the known Angiotensins are established endocrine effectors in the regulation of blood pressure, but they are also known to have other functions locally, in several organs and tissues (paracrine) and at the cellular level (autocrine / intracrine). Angiotensin I has no known effector function but it is an immediate precursor of Angiotensin II. Angiotensin II binds AT1 receptors, which promote vasoconstriction, sodium retention, release of aldosterone, release of Argâ€Âvasopressin, cell proliferation, inflammation, fibrosis, anxiety, and cardiac hypertrophy. Angiotensin A and Angiotensin III, also bind AT1 receptors. Angiotensin (1â€Â7) binds a different receptor called MASâ€Â1 which has opposite effects (vasodilation, natriuresis, antiproliferation, NO release, PGE release, and apoptosis). Angiotensin IV binds yet another receptor called AT4 (IRAP), which promotes increase of blood flow, angiogenesis, and natriuresis, and which has also been implicated in memory formation and in the pathogenesis of Alzheimer’s disease. The peptide LVVâ€Âhemorphinâ€Â7, which is not an angiotensin, also binds the AT4 receptor. There are other angiotensin peptides that Discover more at www.abcam.com 3 INTRODUCTION have been identified, including Ang (1â€Â9), Ang (1â€Â12), Ang V(3â€Â7), as well as several other shorter peptides that have undetermined functions. Angiotensins can be present in very low concentrations in some biological samples. In such cases, dilution of samples to avoid “nonâ€Âspecific†interference by any present factors is not productive because the angiotensin analyte is also diluted to levels far below the minimum detection concentration. Thus, investigators have used several procedures for extracting Angiotensins from biological samples prior to using them for immunoassays. Angiotensins share common sequences, and in some cases they cannot be discriminated by immunoassays. Prior extraction and separation by HPLC may, thus, be required.

Natural and recombinant General Angiotensin I

~ 3 Hours

Mean recovery when spiking into Serum and Plasma = 103%

Serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids