IAPP ELISA Kit (Rat)

ELISA Kit

Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit

10 kDa

islet amyloid polypeptide

Store as indicated in product manual.

NM_012586.2

P12969

Assay Methodology: Quantitative Competitive ELISA

Aviva Systems Biology IAPP ELISA Kit (Rat) (OKEH03223) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Rat Iapp antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Iapp and incubated. The Iapp found in the sample or standards competes with the biotinylated Iapp for limited binding sites on the immobilized anti-Rat Iapp antibody. Excess unbound biotinylated Iapp and sample or standard Iapp is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Iapp captured in the well and inversely proportional to the amount of Iapp which was contained in the sample or standard.