PA1 ELISA Kit (Human)

ELISA Kit

Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit

28 kDa

PAXIP1 associated glutamate rich protein 1

Store as indicated in product manual.

NM_024516.3

Q9BTK6

Assay Methodology: Quantitative Competitive ELISA

Aviva Systems Biology PA1 ELISA Kit (Human) (OKEH03538) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human PA1 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated PA1 and incubated. The PA1 found in the sample or standards competes with the biotinylated PA1 for limited binding sites on the immobilized anti-human PA1 antibody. Excess unbound biotinylated PA1 and sample or standard PA1 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated PA1 captured in the well and inversely proportional to the amount of PA1 which was contained in the sample or standard.

Mean Intra-assay CV%: <= 8.6% (n = 20)
Mean Inter-assay CV%: <= 10.3% (n = 20)