CES5A ELISA Kit (Rat)

ELISA Kit

Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit

64 kDa

carboxylesterase 5A

Store as indicated in product manual.

NM_001012056.1

Q5GRG2

Assay Methodology: Quantitative Competitive ELISA

Aviva Systems Biology CES5A ELISA Kit (Rat) (OKEH03715) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human Ces5a antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Ces5a and incubated. The Ces5a found in the sample or standards competes with the biotinylated Ces5a for limited binding sites on the immobilized anti-human Ces5a antibody. Excess unbound biotinylated Ces5a and sample or standard Ces5a is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Ces5a captured in the well and inversely proportional to the amount of Ces5a which was contained in the sample or standard.