EGR1 ELISA Kit (Bovine)

Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit

57 kDa

early growth response 1

Store as indicated in product manual.

NM_001045875.1

Q29W20

Assay Methodology: Lot dependent. Can either be Quantitative or Sandwich ELISA.

Aviva Systems Biology EGR1 ELISA Kit (Bovine) (OKEH03842) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for EGR1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for EGR1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample EGR1 captured in well.