SOD1 ELISA Kit (Pig)

ELISA Kit

Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit

16 kDa

superoxide dismutase 1

Store as indicated in product manual.

NM_001190422.1

P04178

Assay Methodology: Quantitative Competitive ELISA

Aviva Systems Biology SOD1 ELISA Kit (Pig) (OKEH03853) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-pig SOD1 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated SOD1 and incubated. The SOD1 found in the sample or standards competes with the biotinylated SOD1 for limited binding sites on the immobilized anti-pig SOD1 antibody. Excess unbound biotinylated SOD1 and sample or standard SOD1 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated SOD1 captured in the well and inversely proportional to the amount of SOD1 which was contained in the sample or standard.

Mean Intra-assay CV%: < 6.3% (n = 20)
Mean Inter-assay CV%: < 9.1% (n = 20)