NAMPT ELISA Kit (Rat)

ELISA Kit

Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit

55 KDa

nicotinamide phosphoribosyltransferase

Store as indicated in product manual.

NM_177928.3

Q80Z29

Assay Methodology: Quantitative Sandwich ELISA

Aviva Systems Biology Nampt ELISA Kit (Rat) (OKEH04362) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Nampt has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for Nampt is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Nampt captured in the well.