APOE ELISA Kit (Mouse)

ELISA Kit

Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit, Root Catalog/Research Areas/Neuroscience ELISA Kits

36 KDa

apolipoprotein E

Store as indicated in product manual.

NM_001305819.1

P08226

Assay Methodology: Quantitative Sandwich ELISA

Aviva Systems Biology Apoe ELISA Kit (Mouse) (OKEH04368) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Apoe has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for Apoe is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Apoe captured in the well.

Mean Intra-assay CV%: <= 4.6% (n = 20)
Mean Inter-assay CV%: <= 8.4% (n = 20)