S100a4 ELISA Kit (Rat)

ELISA Kit

Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit

12 kDa

S100 calcium-binding protein A4

Store as indicated in product manual.

NM_012618.2

P05942

Assay Methodology: Quantitative Sandwich ELISA

Aviva Systems Biology S100a4 ELISA Kit (Rat) (OKEH04843) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for S100a4 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for S100a4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample S100a4 captured in well.

Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.