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SPA17 ELISA Kit (Human)

ArtNr OKEH05288
Hersteller AVIVA Systems Biology
Menge 96 Wells
Kategorie
Typ Elisa-Kit
Applikationen ELISA
Specific against Human (Homo sapiens)
Sensitivity 40.6 pg/mL
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias cancer/testis antigen 22;CT22;SP17;SP17-1;Sperm autoantigenic protein 17;sperm protein 17;sperm surface protein Sp17.
Versandbedingung Gekühlt
Lieferbar
Manufacturer - Type
ELISA Kit
Manufacturer - Applications
Enzyme-linked Immunosorbent assay-Sandwich
Manufacturer - Category
Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature
Wet Ice
Gene symbol
SPA17
Gene Fullname
sperm autoantigenic protein 17
Protein size
151
Reconstitution and storage
Store as indicated in product manual.
Description of target
Sperm surface protein Sp17
Nucleotide accession_num
NM_017425.3
Protein accession_num
NP_059121.1
Protein name
Q15506
Manufacturer - Specificity
Natural and recombinant Human Sperm surface protein Sp17
Assay info
Assay Methodology: Quantitative Sandwich ELISA
Kit duration
~3 Hours
Kit principle
Aviva Systems Biology SPA17 ELISA Kit (Human) (OKEH05288) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SPA17 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SPA17 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SPA17 captured in the well.
Kit recovery
Mean recovery when spiking into Serum and Plasma = 99%
Kit reproducibility
Three samples concentrations were measured in replicate within an assay plate and across replicate assays to assess Intra- and Mean Inter-Assay Precision.
Mean Intra-Assay Precision - <7.3%, n = 20
Mean Inter-Assay Precision - <10.7%, n = 20
Sample Type
Serum, plasma and other biological fluids

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 96 Wells
Lieferbar: In stock
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