PIK3CD ELISA Kit (Mouse)

ArtNr	OKEH05498
Hersteller	AVIVA Systems Biology
Menge	96 tests
Kategorie	Oncology By Cell Type Blastoma Carcinoma Glioma Hematology Leukemia Lymphoma Myeloma Melanoma Sarcoma Cell Biology Cell Status Adenocarcinoma Mesothelioma ELISA Autophagy Cardiovascular Research Proliferation Myocardial Infarction ELISA & Immunoassays ELISA Kits Immunoassay Immunostaining
Typ	Elisa-Kit
Applikationen	ELISA
Specific against	Mouse (Murine, Mus musculus)
Sensitivity	0.184 ng/mL
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	2410099E07Rik;2610208K16Rik;AW545373;catalytic phosphatidylinositol 3-kinase delta;p100 delta;p110de;p110delta;phosphatidylinositol 3-kinase catalytic delta polypeptide;phosphatidylinositol 3-kinase catalytic subunit delta;phosphatidylinositol 3-kinase p110 delta;phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit delta;phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit delta isoform;phosphoinositide 3 kinase P110delta;PI3Kdelta;PI3K-delta;PI3-kinase subunit delta;ptdIns-3-kinase subunit delta;ptdIns-3-kinase subunit p110-delta.
Versandbedingung	Gekühlt
Lieferbar
Manufacturer - Type	ELISA Kit
Manufacturer - Applications	Enzyme-linked Immunosorbent assay-Sandwich
Manufacturer - Category	Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature	Wet Ice
Gene symbol	Pik3cd
Gene Fullname	phosphatidylinositol-4, 5-bisphosphate 3-kinase catalytic subunit delta
Protein size	1043
Reconstitution and storage	Store as indicated in product manual.
Description of target	Phosphatidylinositol 4, 5-bisphosphate 3-kinase catalytic subunit delta isoform
Nucleotide accession_num	NM_001164052.1
Protein accession_num	NP_001157524.1
Protein name	O35904
Manufacturer - Specificity	Natural and recombinant Mouse Phosphatidylinositol 4, 5-bisphosphate 3-kinase catalytic subunit delta isoform
Assay info	Assay Methodology: Quantitative Sandwich ELISA
Kit duration	~3 Hours
Kit principle	Aviva Systems Biology Pik3cd ELISA Kit (Mouse) (OKEH05498) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Pik3cd has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Pik3cd is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Pik3cd captured in the well.
Kit recovery	Mean recovery when spiking into Serum and Plasma = 90%
Kit reproducibility	Three samples concentrations were measured in replicate within an assay plate and across replicate assays to assess Intra- and Mean Inter-Assay Precision. Mean Intra-Assay Precision - <6.8%, n = 20 Mean Inter-Assay Precision - <8.4%, n = 20
Sample Type	Serum, plasma and other biological fluids

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OKEH05498.pdf

OKEH05498-datasheet.pdf

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Menge: 96 tests

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