TSPAN7 ELISA Kit (Human)

Enzyme-linked Immunosorbent assay-Sandwich

Wet Ice

tetraspanin 7

Store as indicated in product manual.

NM_004615.3

P41732

Assay Methodology: Quantitative Sandwich ELISA

Aviva Systems Biology TSPAN7 ELISA Kit (Human) (OKEH05775) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TSPAN7 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for TSPAN7 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TSPAN7 captured in well.

Serum, plasma and other biological fluids