NT5E ELISA Kit (Mouse)

Enzyme-linked Immunosorbent assay-Sandwich

Wet Ice

5' nucleotidase, ecto

Store as indicated in product manual.

NM_011851.4

Q61503

Assay Methodology: Quantitative Sandwich ELISA

Aviva Systems Biology Nt5e ELISA Kit (Mouse) (OKEH05923) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Nt5e has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Nt5e is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Nt5e captured in well.

Three samples concentrations were measured in replicate within an assay plate and across replicate assays to assess Intra- and Mean Inter-Assay Precision.
Mean Intra-Assay Precision - <8.2%, n = 20
Mean Inter-Assay Precision - <9.6%, n = 20