NTAN1 ELISA Kit (Mouse)

ELISA Kit

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Ntan1

Store as indicated in product manual.

NM_134250

Q64311

Assay Methodology: Quantitative Sandwich ELISA

Aviva Systems Biology Ntan1 ELISA Kit (Mouse) (OKEH06503) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Ntan1 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Ntan1 and incubated. The Ntan1 found in the sample or standards competes with the biotinylated Ntan1 for limited binding sites on the immobilized anti-Ntan1 antibody. Excess unbound biotinylated Ntan1 and sample or standard Ntan1 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Ntan1 captured in the well and inversely proportional to the amount of Ntan1 which was contained in the sample or standard.

Three samples concentrations were measured in replicate within an assay plate and across replicate assays to assess Mean Intra- and Mean Inter-Assay Precision.
Mean Intra-Assay Precision - <= 6.8% (n = 20)
Mean Inter-Assay Precision - <= 8.7% (n = 20)