HILPDA ELISA Kit (Mouse)

Enzyme-linked Immunosorbent assay-Sandwich

Wet Ice

hypoxia inducible lipid droplet associated

Hypoxia-inducible lipid droplet-associated protein

NP_076005

Assay Methodology: Quantitative Sandwich ELISA

Aviva Systems Biology Hilpda ELISA Kit (Mouse) (OKEH06764) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Hilpda has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Hilpda is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Hilpda captured in the well.

Intra-Assay Reproducibility CV% < 8.8% (n=20)< br>Inter-Assay Reproducibility CV% < 10.6% (n=24)