Proinsulin ELISA Kit (Pig)

ELISA Kit

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Proinsulin

Proinsulin

Assay Methodology: Quantitative Sandwich ELISA

Aviva Systems Biology Proinsulin ELISA Kit (Pig) (OKEH07377) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Proinsulin has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Proinsulin is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Proinsulin captured in well.

Mean Intra-assay CV%: <=6.6% (n = 20)
Mean Inter-assay CV%: <=7.5% (n = 20)