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UGT1A4 ELISA Kit (Human)

ArtNr OKEH07890
Hersteller AVIVA Systems Biology
Menge 96 tests
Kategorie
Typ Elisa-Kit
Applikationen ELISA
Specific against Human (Homo sapiens)
Sensitivity 26pg/mL
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias bilirubin UDP-glucuronosyltransferase isozyme 2;Bilirubin-specific UDPGT isozyme 1;bilirubin-specific UDPGT isozyme 2;GNT1;hUG-BR1;HUG-BR2;UDP glucuronosyltransferase 1 family,polypeptide A4;UDP glycosyltransferase 1 family,polypeptide A4;UDP-glucuronosyltransferase 1 family polypeptide A4s;UDP-glucuronosyltransferase 1-1;UDP-glucuronosyltransferase 1-4;UDP-glucuronosyltransferase 1-A;UDP-glucuronosyltransferase 1A1;UDP-glucuronosyltransferase 1A4;UDP-glucuronosyltransferase 1-D;UDPGT;UDPGT 1-1;UDPGT 1-4;UGT1;UGT1.1;UGT1.4;UGT1-01;UGT1-04;UGT1A;UGT-1A;UGT1A1;UGT1A4S;UGT1D;UGT-1D.
Versandbedingung Gekühlt
Lieferbar
Manufacturer - Type
ELISA Kit
Manufacturer - Applications
Enzyme-linked Immunosorbent assay-Sandwich
Manufacturer - Category
Root Catalog/Products/Kit, Root Catalog/Products/ELISA Kits
Shipping Temperature
Wet Ice
Gene symbol
UGT1A4
Gene Fullname
UDP glucuronosyltransferase family 1 member A4
Reconstitution and storage
Store as indicated in product manual.
Description of target
UDP-glucuronosyltransferase 1-4
Nucleotide accession_num
NM_007120.2
Protein accession_num
NP_009051.1
Protein name
P22310
Manufacturer - Specificity
Natural and recombinant Human UDP-glucuronosyltransferase 1-4
Assay info
Assay Methodology: Quantitative Sandwich ELISA
Kit linearity
Sample - 1:2 - 1:4 - 1:8 - 1:16serum(n=5) - 97-107% (n = 20) - 86-96% (n = 20) - 103-113% (n = 20) - 105-115% (n = 20)EDTA plasma(n=5) - 95-107% (n = 20) - 102-112% (n = 20) - 109-119% (n = 20) - 102-112% (n = 20)heparin plasma(n=5) - 99-107% (n = 20) - 110-120% (n = 20) - 88-98% (n = 20) - 83-93% (n = 20)
Kit principle
Aviva Systems Biology UGT1A4 ELISA Kit (Human) (OKEH07890) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for UGT1A4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for UGT1A4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample UGT1A4 captured in well.
Kit recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 92% (n = 20)
Kit reproducibility
Mean Intra-assay CV%: <=8.3% (n = 20)
Mean Inter-assay CV%: <=9.6% (n = 20)

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 96 tests
Lieferbar: In stock
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