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ARHGEF7 ELISA Kit (Rat)

ArtNr OKEH08018
Hersteller AVIVA Systems Biology
Menge 96 tests
Kategorie
Typ Elisa-Kit
Applikationen ELISA
Specific against Rat (Rattus norvegicus)
Sensitivity 0.052ng/mL
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias beta-Pix;P85spr;Pak3bp;PAK-interacting exchange factor beta;Rho guanine nucleotide exchange factor (GEF) 7;rho guanine nucleotide exchange factor 7.
Versandbedingung Gekühlt
Lieferbar
Manufacturer - Type
ELISA Kit
Manufacturer - Applications
Enzyme-linked Immunosorbent assay-Sandwich
Manufacturer - Category
Root Catalog/Products/Kit, Root Catalog/Products/ELISA Kits
Shipping Temperature
Wet Ice
Gene symbol
Arhgef7
Gene Fullname
Rho guanine nucleotide exchange factor 7
Reconstitution and storage
Store as indicated in product manual.
Description of target
Rho guanine nucleotide exchange factor 7
Nucleotide accession_num
NM_001113522.1
Protein accession_num
NP_001106994.1
Protein name
O55043
Manufacturer - Specificity
Natural and recombinant Rat Rho guanine nucleotide exchange factor 7
Assay info
Assay Methodology: Quantitative Sandwich ELISA
Kit linearity
Sample - 1:2 - 1:4 - 1:8 - 1:16serum(n=5) - 86-95% (n = 20) - 100-110% (n = 20) - 87-97% (n = 20) - 81-90% (n = 20)EDTA plasma(n=5) - 96-108% (n = 20) - 95-105% (n = 20) - 92-102% (n = 20) - 109-120% (n = 20)heparin plasma(n=5) - 104-114% (n = 20) - 95-104% (n = 20) - 92-104% (n = 20) - 113-123% (n = 20)
Kit principle
Aviva Systems Biology ARHGEF7 ELISA Kit (Rat) (OKEH08018) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ARHGEF7 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ARHGEF7 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ARHGEF7 captured in well.
Kit recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 102% (n = 20)
Kit reproducibility
Mean Intra-assay CV%: <=5.2% (n = 20)
Mean Inter-assay CV%: <=8.5% (n = 20)

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 96 tests
Lieferbar: In stock
lieferbar

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