PLA2G4A ELISA Kit (Bovine)

Enzyme-linked Immunosorbent assay-Sandwich

Wet Ice

phospholipase A2 group IVA

Cytosolic phospholipase A2

NP_001069332.1

Natural and recombinant Bovine Cytosolic phospholipase A2

Aviva Systems Biology PLA2G4A ELISA Kit (Bovine) (OKEH08397) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PLA2G4A has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PLA2G4A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PLA2G4A captured in well.

Mean Intra-assay CV%: <=4.5% (n = 20)
Mean Inter-assay CV%: <=5.2% (n = 20)