CEBPD ELISA Kit (Human)

Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit

CEBPD

Transcription activator that recognizes two different DNA motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers. Important transcription factor regulating the expression of genes involved in immune and inflammatory responses. Transcriptional activator that enhances IL6 transcription alone and as heterodimer with CEBPB.

NP_005186

Assay Methodology: Quantitative Sandwich ELISA

Aviva Systems Biology CEBPD ELISA Kit (Human) (OKEI00146) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CEBPD has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CEBPD is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of a luminol substrate which is catalyzed by the HRP to produce light emission. The light emission is read by a luminometer (or photo-multiplier equipped instrument) and the intensity of the emitted light is proportional to the amount of sample CEBPD

Serum, Plasma, Biological Fluids