HSPA9 ELISA Kit (Rat)

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HSPA9

Chaperone protein which is implicated in the control of cell proliferation and cellular aging. Plays a role in the erythropoiesis process.

Assay Methodology: Quantitative Sandwich ELISA

Aviva Systems Biology Hspa9 ELISA Kit (Rat) (OKEI00784) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Hspa9 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Hspa9 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of a luminol substrate which is catalyzed by the HRP to produce light emission. The light emission is read by a luminometer (or photo-multiplier equipped instrument) and the intensity of the emitted light is proportional to the amount of sample Hspa9

Serum, Plasma, Biological Fluids