TRIM26 ELISA Kit (Human)

ELISA Kit

Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit

AFP

Store as indicated in product manual.

NM_001242783

Alpha-fetoprotein

Component: Amount
TRIM26 Microplate: 96 Wells (12 x 8 Well strips)
TRIM26 Lyophilized Standard: 2 x 20 ng
100X TRIM26 Biotinylated Detector Anitbody: 1 x 120 uL
100X Avidin-HRP Conjugate: 1 x 120 uL
Standard Diluent: 1 x 20 mL
Detector Antibody Diluent: 1 x 12 mL
Conjugate Diluent: 1 x 12 mL
25X Wash Buffer: 1 x 20 mL
TMB Substrate: 1 x 10 mL
Stop Solution: 1 x 10 mL

~ 3 Hours

Aviva Systems Biology TRIM26 ELISA Kit (Human) (OKWB00154) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TRIM26 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TRIM26 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TRIM26 captured in the well.

Mean Intra-Assay Precision <10%
Mean Inter-Assay Precision <12%