ULK1 ELISA Kit (Human )

Enzyme-linked Immunosorbent assay-Sandwich

Wet Ice

unc-51 like autophagy activating kinase 1

Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation. May also phosphorylate SESN2 and SQSTM1 to regulate autophagy (PubMed:25040165).

NP_003556

Assay Type: Quantitative Sandwich ELISA

Colorimetric, OD 450 nm

Sample Type - 1:2 Dilution - 1:4 Dilution - 1:8 Dilution - 1:16 Dilution
Serum (n=5) - 88-104% - 86-103% - 88-104% - 88-98%
EDTA Plasma (n=5) - 84-95% - 82-101% - 84-92% - 85-101%
Heparin Plasma (n=5) - 80-99% - 81-98% - 81-97% - 81-99%

Sample Type - Range - Mean
Serum (n=5) - 86-97 - 92
EDTA Plasma (n=5) - 90-105 - 98
Heparin Plasma (n=5) - 87-103 - 96

Serum, Plasma, Tissue Homogenates and Other Biological Fluids