MUL1 ELISA Kit (Human)

Enzyme-linked Immunosorbent assay-Sandwich

Wet Ice

nuclear factor kappa B subunit 1

NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and RelB-p50 complexes are transcriptional activators. The NF-kappa-B p50-p50 homodimer is a transcriptional repressor, but can act as a transcriptional activator when associated with BCL3. NFKB1 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p105 and generation of p50 by a cotranslational processing. The proteasome-mediated process ensures the production of both p50 and p105 and preserves their independent function, although processing of NFKB1/p105 also appears to occur post-translationally. p50 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. In a complex with MAP3K8, NFKB1/p105 represses MAP3K8-induced MAPK signaling; active MAP3K8 is released by proteasome-dependent degradation of NFKB1/p105.

NP_078820

Assay Type: Quantitative Sandwich ELISA

Colorimetric, OD 450 nm

Sample Type - 1:2 Dilution - 1:4 Dilution - 1:8 Dilution - 1:16 Dilution
Serum (n=5) - 86-100% - 91-102% - 85-100% - 85-104%
EDTA Plasma (n=5) - 88-99% - 85-100% - 84-96% - 89-101%
Heparin Plasma (n=5) - 80-97% - 80-89% - 80-96% - 82-100%

Sample Type - Range - Mean
Serum (n=5) - 87-100 - 94
EDTA Plasma (n=5) - 86-101 - 93
Heparin Plasma (n=5) - 87-103 - 95

Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids