ATXN1 ELISA Kit (Mouse)

ELISA Kit

Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit

Atxn1

Store as indicated in product manual.

NM_001199304

Ataxin-1

Component: Amount
Atxn1 Microplate: 96 Wells (12 x 8 Well strips)
Atxn1 Lyophilized Standard: 2 x 1 ng
100X Atxn1 Biotinylated Detector Anitbody: 1 x 120 uL
100X Avidin-HRP Conjugate: 1 x 120 uL
Standard Diluent: 1 x 20 mL
Detector Antibody Diluent: 1 x 12 mL
Conjugate Diluent: 1 x 12 mL
25X Wash Buffer: 1 x 20 mL
TMB Substrate: 1 x 10 mL
Stop Solution: 1 x 10 mL

~ 3 Hours

Aviva Systems Biology Atxn1 ELISA Kit (Mouse) (OKWB00241) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Atxn1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Atxn1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Atxn1 captured in the well.

Mean Intra-Assay Precision <10%
Mean Inter-Assay Precision <12%