ACKR4 ELISA Kit (Mouse)

Enzyme-linked Immunosorbent assay-Sandwich

Wet Ice

atypical chemokine receptor 4

Atypical chemokine receptor that controls chemokine levels and localization via high-affinity chemokine binding that is uncoupled from classic ligand-driven signal transduction cascades, resulting instead in chemokine sequestration, degradation, or transcytosis. Also known as interceptor (internalizing receptor) or chemokine-scavenging receptor or chemokine decoy receptor. Acts as a receptor for chemokines CCL2, CCL8, CCL13, CCL19, CCL21 and CCL25. Chemokine-binding does not activate G-protein-mediated signal transduction but instead induces beta-arrestin recruitment, leading to ligand internalization. Plays an important role in controlling the migration of immune and cancer cells that express chemokine receptors CCR7 and CCR9, by reducing the availability of CCL19, CCL21, and CCL25 through internalization. Negatively regulates CXCR3-induced chemotaxis. Regulates T-cell development in the thymus and inhibits spontaneous autoimmunity.

NP_663746

Assay Type: Quantitative Sandwich ELISA

Colorimetric, OD 450 nm

Sample Type - 1:2 Dilution - 1:4 Dilution - 1:8 Dilution - 1:16 Dilution
Serum (n=5) - 86-102% - 87-100% - 85-102% - 103-105%
EDTA Plasma (n=5) - 86-100% - 88-93% - 84-101% - 84-97%
Heparin Plasma (n=5) - 86-98% - 83-90% - 81-100% - 80-100%

Sample Type - Range - Mean
Serum (n=5) - 86-99 - 92
EDTA Plasma (n=5) - 95-102 - 100
Heparin Plasma (n=5) - 89-98 - 95

Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids