NLRP1A ELISA Kit (Mouse)

Enzyme-linked Immunosorbent assay-Sandwich

Wet Ice

NLR family, pyrin domain containing 1A

As a potential sensor component of the NLRP1 inflammasome, plays a crucial role in innate immunity and inflammation. In response to pathogens and other damage-associated signals, initiates the formation of the inflammasome polymeric complex, made of Nlrp1b, CASP1, and possibly PYCARD. Recruitment of proCASP1 to the inflammasome promotes its activation and CASP1-catalyzed IL1B and IL18 maturation and secretion in the extracellular milieu. Activation of NLRP1 inflammasome is also required for HMGB1 secretion. The active cytokines and HMGB1 stimulate inflammatory responses. Inflammasomes can also induce pyroptosis, an inflammatory form of programmed cell death (By similarity) (PubMed:23219391). When activated in the bone marrow, induces the pyroptosis of hematopoietic stem cells and progenitor cells of both myeloid and lymphoid lineages, hence allowing the removal of damaged cells, and the release of IL1B, which induces granulopoiesis (PubMed:23219391). Binds ATP (By similarity).

NP_001004142

Assay Type: Quantitative Sandwich ELISA

Colorimetric, OD 450 nm

Sample Type - 1:2 Dilution - 1:4 Dilution - 1:8 Dilution - 1:16 Dilution
Serum (n=5) - 96-105% - 85-103% - 85-103% - 93-105%
EDTA Plasma (n=5) - 84-99% - 86-99% - 82-97% - 82-92%
Heparin Plasma (n=5) - 80-96% - 84-99% - 83-96% - 90-99%

Sample Type - Range - Mean
Serum (n=5) - 87-103 - 95
EDTA Plasma (n=5) - 92-101 - 96
Heparin Plasma (n=5) - 85-96 - 92

Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids