MAPK8IP1 ELISA Kit (Rat)

Enzyme-linked Immunosorbent assay-Sandwich

Wet Ice

mitogen-activated protein kinase 8 interacting protein 1

The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. Required for JNK activation in response to excitotoxic stress. Cytoplasmic MAPK8IP1 causes inhibition of JNK-regulated activity by retaining JNK in the cytoplasm and thus inhibiting the JNK phosphorylation of c-Jun. May also participate in ApoER2-specific reelin signaling. Directly, or indirectly, regulates GLUT2 gene expression and beta-cell function. Appears to have a role in cell signaling in mature and developing nerve terminals. May function as a regulator of vesicle transport, through interactions with the JNK-signaling components and motor proteins. Functions as an anti-apoptotic protein and whose level seems to influence the beta-cell death or survival response (By similarity).

NP_446229

Assay Type: Quantitative Sandwich ELISA

Colorimetric, OD 450 nm

Sample Type - 1:2 Dilution - 1:4 Dilution - 1:8 Dilution - 1:16 Dilution
Serum (n=5) - 95-99% - 87-104% - 90-101% - 90-105%
EDTA Plasma (n=5) - 83-101% - 94-101% - 82-96% - 87-100%
Heparin Plasma (n=5) - 80-98% - 87-100% - 80-97% - 80-96%

Sample Type - Range - Mean
Serum (n=5) - 87-103 - 95
EDTA Plasma (n=5) - 86-102 - 93
Heparin Plasma (n=5) - 91-104 - 96

Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids