IL12B ELISA Kit (Rabbit)

ELISA Kit

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IL12B

Store as indicated in product manual.

XM_002710347

Interleukin-12 subunit beta

Component: Amount
IL12b Microplate: 96 Wells (12 x 8 Well strips)
IL12b Lyophilized Standard: 2 x 2 ng
100X IL12b Biotinylated Detector Anitbody: 1 x 120 uL
100X Avidin-HRP Conjugate: 1 x 120 uL
Standard Diluent: 1 x 20 mL
Detector Antibody Diluent: 1 x 12 mL
Conjugate Diluent: 1 x 12 mL
25X Wash Buffer: 1 x 20 mL
TMB Substrate: 1 x 10 mL
Stop Solution: 1 x 10 mL

~ 3 Hours

Aviva Systems Biology IL12b ELISA Kit (Rabbit) (OKWB00410) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IL12b has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IL12b is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IL12b captured in the well.

Mean Intra-Assay Precision <10%
Mean Inter-Assay Precision <12%