HSP70 ELISA Kit (Sheep)

Enzyme-linked Immunosorbent assay-Sandwich

Wet Ice

heat shock 70kDa protein 1A

NM_001267874

Heat shock protein 70

Component: Amount
Hsp70 Microplate: 96 Wells (12 x 8 Well strips)
Hsp70 Lyophilized Standard: 2 x 200 ng
100X Hsp70 Biotinylated Detector Anitbody: 1 x 120 uL
100X Avidin-HRP Conjugate: 1 x 120 uL
Standard Diluent: 1 x 20 mL
Detector Antibody Diluent: 1 x 12 mL
Conjugate Diluent: 1 x 12 mL
25X Wash Buffer: 1 x 20 mL
TMB Substrate: 1 x 10 mL
Stop Solution: 1 x 10 mL

~ 3 Hours

Aviva Systems Biology Hsp70 ELISA Kit (Sheep) (OKWB00440) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Hsp70 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Hsp70 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Hsp70 captured in the well.

Mean Intra-Assay Precision <10%
Mean Inter-Assay Precision <12%