Clusterin ELISA Kit (Rat)

ELISA Kit

Root Catalog/Products/ELISA Kits, Root Catalog/Products/ELISA Kits/Blood Proteins

CLU

In Solution

Functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. When secreted, protects cells against apoptosis and against cytolysis by complement. Intracellular forms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Promotes apoptosis when in the nucleus. Inhibits apoptosis when associated with the mitochondrial membrane by interference with BAX-dependent release of cytochrome c into the cytoplasm. Plays a role in the regulation of cell proliferation.

Rat

Component: Amount
Anti-Rat Clusterin (Apolipoprotein J) Microplate: 96 Wells (12 x 8 Well Strips)
20X Diluent Concentrate: 50 mL
20X Wash Solution: 50 mL
100X Enzyme-Antibody Conjugate: 150 uL
Chromogen-Substrate Solution: 12 mL
Stop Solution: 12 mL
Rat Clusterin (Apolipoprotein J) Calibrator: 1

100 Minutes

Serum, plasma and other biological fluids