Vergleich

NORMAL GOAT SERUM (NGS)

ArtNr OORA01661
Hersteller AVIVA Systems Biology
Menge 10 ml
Kategorie
Typ Serum
Format Lyophilized
Applikationen WB, FC, IF, IP, IHC, ELISA, CHIP, SDS-PAGE
Specific against other
Konjugat/Tag Unconjugated
ECLASS 10.1 32160802
ECLASS 11.0 32160802
UNSPSC 12352207
Alias blocking goat serum,blocking grade,10% NGS
Versandbedingung Gekühlt
Lieferbar
Manufacturer - Type
Others
Manufacturer - Applications
Chromatin Immunoprecipitation, Enzyme-linked immunosorbent assay, Flow cytometry, Fluorescence-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Sodium sodecyl sulfate - polyacrylamide gel electrophoresis, Western blot
Shipping Temperature
Wet Ice
Manufacturer - Application Additional Information
Goat Serum blocking reagent or NGS is ideal for blocking procedures such as WB, ELISA and immunochemistry to prevent nonspecific binding.
Product format
Lyophilized 0.02M Potassium Phosphate, 0.15M Sodium Chloride (pH 7.2) with 0.01% (w/v) sodium azide and 0.01% (w/v) gentamicin sulfate
Reconstitution and storage
Store Normal Goat Serum (NGS) at 4°C prior to restoration. Restore with 10 ml of deionized water (or equivalent). For extended storage aliquot contents and freeze Goat Serum at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.
Description of target
Normal Goat Serum can be used as a blocking agent to treat plastic surfaces, membrane or tissue after they have been sensitized with primary antibody or antigen. It provides an alternative to bovine serum albumin (BSA) and non-fat dry milk. It is effective in reducing nonspecific binding of proteins to reaction surfaces, thereby maximizing signal-to-noise ratio. This blocking agent is recommended for use in immunoassays where the primary antibody was produced in goat, as a source of non-specific serum protein or on tissue for immunohistochemical applications.
Purification
Goat Serum blocking reagent or NGS was prepared from normal serum by a multi-step process which includes delipidation and selective precipitation. Assay by immunoelectrophoresis resulted in a multiple precipitin arcs against anti-Goat Serum. Normal Goat Serum was obtained from non-immunized healthy goats.
Concentration
105 mg/ml

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 10 ml
Lieferbar: In stock
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