BLOTTO Immunoanalytical Grade (Non-Fat Dry Milk)

Biological activity assay, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunoprecipitation, Western blot

Use BLOTTO whenever procedures call for immunoanalytical grade non-fat dry milk. Alternative blocking agents such as BLOTTO A (solution of TBS, 5% non-fat dry milk and 0.05% Tween-20) and BLOTTO B (solution of TBS, 1% non-fat dry milk, 1% BSA and 0.05% Tween-20) are also available. BLOTTO A is a convenient general purpose blocking agent. BLOTTO B is recommended for blocking when phospho-specific antibodies are used. BLOTTO A and BLOTTO B provide Tween-20 in a separate vial in liquid form. Users should consider the addition of protease inhibitors and phosphatase inhibitors when required. Typically 50 mM NaF can be added to BLOTTO solutions to inhibit phosphatase activity.

Reconstitute with deionized water (or equivalent). Store container at room temperature prior to opening. After reconstitution, use blocking buffers immediately. Dilute solutions may be stored at 4°C for up to four (4) days. Solutions containing BLOTTO may be frozen. Expiration date of dry material is one (1) year from date of opening.