Hersteller |
AVIVA Systems Biology
|
Kategorie |
|
Typ |
Proteins |
Specific against |
other |
Format |
Lyophilized from a concentrated solution (1mg/ml) contains 150mM NaCl, and 50mM sodium phosphate, pH 7.4. Physical appearance: Sterile Filtered White lyophilized (freeze-dried) powder. |
Menge |
10ug |
Host |
E.coli |
ArtNr |
OPPA00702 |
eClass 6.1 |
34160400 |
eClass 9.0 |
42020190 |
Lieferbar |
|
Description |
TRAIL/APO 2 Ligand Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 168 amino acids (Met+Arg115-Gly281). |
Gene symbol |
TRAIL |
Protein name |
Tumor necrosis factor ligand superfamily member 10 |
Molecular weight |
ca.21 kDa |
Product format |
Lyophilized from a filtered (0.2μ m) solution containing 20mM Tris-HCl pH 8.0 and 150mM NaCl Physical Appearance: Sterile filtered white powder |
Gene id |
8743 |
Reconstitution and storage |
It is recommended to reconstitute the lyophilized TRAIL in sterile 18MΩ -cm H2O not less than 100μ g/ml, which can then be further diluted to other aqueous solutions.
Lyophilized APO 2 Ligand although stable at room temperature for 3 weeks, should be stored desiccated below -18ACC. Upon reconstitution TRAIL should be stored at 4° Cbetween 2-7 days and for future use below -18° C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
Description of target |
TNF-related apoptosis-inducing ligand (TRAIL) is a ligand molecule which induces apoptosis. It is a type II transmembrane protein with homology to other members of the tumor necrosis factor family. In humans, the gene that encodes for TRAIL is located at chromosome 3q26. TRAIL binds to the death receptors, DR4 and DR5. The process of apoptosis is caspase-8-dependent. This protein preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. |
Purification |
The sTRAIL is purified by proprietary chromatographic techniques. |
Biological activity |
The activity is determined by the cytolysis of Murine L929 cells in the presence of Actinomycin D, ED50 for this effect is less than 2ng/ml, corresponding to a specific activity of 5, 000, 000 IU/mg. |
Purity |
Greater than 95.0% as determined by: (a) Analysis by RP-HPLC (b) Analysis by SDS-PAGE |
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