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TaqDNA Protein

ArtNr OPPA00828
Hersteller AVIVA Systems Biology
Menge 1,000 Units
Kategorie
Typ Proteins Recombinant
Format Liquid
Specific against other
Host E.coli, Recombinant
Purity Greater than 95.0% as determined by SDS-PAGE.
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Alias DNA polymerase I thermostable,EC 2.7.7.7,Taq polymerase 1,TaqDNA,Taq DNA Polymerase Recombinant
Similar products TaqDNA
Versandbedingung Gekühlt
Lieferbar
Manufacturer - Type
Protein
Manufacturer - Category
Root Catalog/Products/Recombinant Proteins
Manufacturer - Host
Recombinant e.coli contains Thermus aquaticus polymerase gene
Shipping Temperature
Wet Ice
Description
Taq DNA Polymerase(a) is a thermostable enzyme of approximately 95 kDa isolated from Thermus aquaticus. This unmodified enzyme replicates DNA at 74° C and exhibits a half-life of 40 minutes at 95° C. The enzyme catalyzes the polymerization of nucleotides into duplex DNA in the 5´~3´ direction in the presence of magnesium and also possesses a 5´~3´ exonuclease activity. Taq DNA Polymerase is recommended for use in PCR but is not recommended for use in DNA sequencing reactions.
Gene symbol
TAQDNA
Product format
Liquid 20mM Tris-HCl (pH 8.0), 100mM KCl, 0.1mM EDTA, 1mM DTT, 50% glycerol, 0.5% NP40, 0.5% Tween-20
Reconstitution and storage
Stable for 5 days at 10°C, for longer period of time store at -20°C.
Description of target
Recombinant Taq DNA Polymerase
Protein name
DNA polymerase I, thermostable
Biological activity
One unit is defined as the amount of enzyme required to catalyze the incorporation of 10nmol of dNTP into acid-insoluble material in 30 minutes at 74° C. The reaction conditions are: 50mM Tris-HCl (pH 9.0 at 25° C), 50mM NaCl, 5mM MgCl2, 200 um each of dATP, dCTP, dGTP, dTTP (a mix of unlabeled and [3H]dTTP), 10 ug activated calf thymus DNA and 0.1mg/ml BSA in a final volume of 50ul.
Concentration
20 IU/uL

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 1,000 Units
Lieferbar: In stock
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