HRP - Horseradish Peroxidase Protein (OPPA00841)

Hersteller AVIVA Systems Biology
Kategorie
Typ Proteins
Specific against other
Format Sterile Filtered red-brown lyophilized powder.
Menge 20mg
ArtNr OPPA00841
eClass 6.1 34160400
eClass 9.0 42020190
Lieferbar
Gene symbol
HRP
Molecular weight
44 kDa
Product format
Lyophilized
Physical Appearance: Sterile filtered red-brown powder
Reconstitution and storage
It is recommended to reconstitute the lyophilized HRP in sterile 18MΩ -cm H2O not less than 100 μ g/ml or more than 10 mg/ml solutions.
Lyophilized HRP although stable at room temperature for 3 weeks, should be stored desiccated below -18° C. Upon reconstitution HRP should be stored at 4° C between 2-7 days and for future use below -18° C.
Please prevent freeze-thaw cycles.
Description of target
The enzyme horseradish peroxidase, found in horseradish, is used extensively in molecular biologyand in antibody amplification and detection, among other things. For example, "In recent years the technique of marking neurons with the enzyme horseradish peroxidase (HRP) has become a major tool. In its brief history, this method has probably been used by more neurobiologists than have used the Golgi stainsince its discovery in 1870." Horseradish peroxidase is also highly used in techniques such as Western Blot and ELISA.
HRP is widely used as an enzymatic label in immunoassays. Usually, the enzyme is coupled to antibodies, lectins or haptens. Coupling to antibodies etc. may be performed through the carbohydrate side chains of the HRP.
Purification
Purified by affinity chromatography, which results in an enzyme of high specific activity and purity.
Biological activity
276 U/mg (25° C, guaiacol as the hydrogen donor, pH-7 and H2O2 as substrates).
Purity
(A403/A275) = RZ: 3.0

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Menge: 20mg
Lieferbar: In stock
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