PKC-a Protein (OPPA01132)

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Hersteller AVIVA Systems Biology
Kategorie
Typ Proteins
Specific against other
Format PKC-a is supplied at a of 0.3mg/ml in 50mM Tris, pH 7.4, 0.1M NaCl, 20% glycerol, 1mM DTT, 0.1mM EDTA, 0.2mM PMSF and 0.03% Brij-35.
Menge 2ug
Host Insect cells
ArtNr OPPA01132
eClass 6.1 34160400
eClass 9.0 42020190
Lieferbar
Gene symbol
PKC-a
Protein name
Protein kinase C alpha type
Molecular weight
76.7 kDa
Product format
Liquid. 20mM HEPES pH 7.5, 250mM NaCl, 50% glycerol, 2mM EDTA, 2mM EGTA, 0.05% Triton X-100, and 5mM DTT.
Gene id
5578
Reconstitution and storage
Store at 4C if entire vial will be used within 2-4 weeks.
Store, frozen at -20C for longer periods of time.
Avoid multiple freeze-thaw cycles.
Description of target
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes.
Purification
PKC-a is purified by proprietary chromatographic techniques.
Biological activity
1570 nmole of phosphate transferred to Histone H1 substrate per minute per mg of total protein at 30C. Activity determined at a final protein concentration of 0.5 to 2ug/ml.
7200- 9000 units/mg. One unit of PKC-a activity transfers 1 nmole of phosphate to a PKC substrate peptide (myelin basic protein 4-14) per minute at 30C. Recombinant human active PKC-a also phosphorylates full length myelin basic protein, histone, MARKS, and GSK-3b. Activity requires incubation with lipid activators, and may vary depending on the substrate and reaction conditions.
Purity
Greater than 95% as determined by SDS-PAGE.
Drywet
Wet Ice

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Menge: 2ug
Lieferbar: In stock
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