TXN1 Yeast Protein (OPPA01248)

Hersteller AVIVA Systems Biology
Kategorie
Typ Proteins
Specific against other
Format Each mg of protein contains 20mM phosphate buffer pH 7.4. Physical appearance: Sterile Lyophilized Powder.
Menge 5ug
Host E.coli
ArtNr OPPA01248
eClass 6.1 34160400
eClass 9.0 42020190
Lieferbar
Gene symbol
yTXN1
Protein name
Thioredoxin-1
Product format
Each mg of protein contains 20mM phosphate buffer pH 7.4. Physical appearance: Sterile Lyophilized Powder.
Gene id
850732
Reconstitution and storage
It is recommended to reconstitute the lyophilized TRX in sterile 18M Omega -cm H2O. TRX although stable at 4C for 3 weeks, should be stored desiccated below -18C. Please prevent freeze thaw cycles.
Description of target
Thioredoxins are small disulphide-containing redox proteins (within the conserved Cys-Gly-Pro-Cys active site) that have been found in all the kingdoms of living organisms. Thioredoxin contains a single disulfide active site and serves as a general protein disulphide oxidoreductase. Thioredoxins are involved in the first unique step in DNA synthesis. It interacts with a broad range of proteins by a redox mechanism based on reversible oxidation of two cysteine thiol groups to a disulphide, accompanied by the transfer of two electrons and two protons. The net result is the covalent interconversion of a disulphide and a dithiol. It has been suggested that thioredoxin may catalyze the formation of correct disulfides during protein folding because of its ability to act as an efficient oxidoreductant. Trx also provides control over a number of transcription factors affecting cell proliferation and death through a mechanism referred to as redox regulation.
Thioredoxin Yeast Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain having a molecular mass of 12.6kDa.
Biological activity
TRX activity is assayed by measuring the change in absorbance at 650 nm at 25C using 0.13 uM bovine insulin containing 0.33mM DTT (pH 6.5).The specific activity was found to be 3IU/mg.
Purity
Greater than 95.0% as determined by SDS-PAGE.

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Menge: 5ug
Lieferbar: In stock
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