CALB2 Protein

Root Catalog/Products/Recombinant Proteins

This protein can be used for immunoblots, absorption experiments in immunohistochemistry, radioimmunoassay and intracellular injection.For adsorption we suggest the following procedureA- Dilute 1 ul of the antiserum against calretinin in 5 ml of the usual buffer for immunohistochemistry (final dilution 1:5'000).B- Add 1 ug of recombinant protein to 1ml of the diluted antibody solution and mix well.C- Incubate for at least 6 hours in the cold.D- Apply to tissue-sections and incubate for 3 days.E - Complete the immunohistochemical reaction as usual (biotinylated second antibody, ABC-complex, DAB).As a result, the immunostaining should be strongly reduced or even completely prevented.

Recombinant

Lyophilized CABP29K although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution CABP29K should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0. 1% HSA or BSA). Please prevent freeze-thaw cycles.

NP_001731.2