PAF-AH Protein (OPPB00035)

Platelet Activating Factor (PAF) is a biologically active phospholipid, which exerts primarily proinflammatory activities by specifically signaling through G-protein-coupled receptors on platelets, neutrophils, and monocytes. Platelet Activating Factor Acetylhydrolase (PAF-AH) is a secreted protein that mediates PAF activity by specifically catalyzing hydrolysis of the “sn2” ester bond, resulting in the conversion of PAF to the biologically inactive lyso-PAF. PAF-AH can also interact with LDL particles to induce the hydrolysis of LDL associated, oxidized phospholipids, generating lysophosphatidylcholine (lyso-PC) and other lysophospholipids. Recombinant PAF-AH is a 420 amino acid glycoprotein which migrates with an apparent molecular mass of 47-55 kDa by SDS-PAGE analysis.

Preparation: Measured by its ability to cleave a PAF analog in a chromogenic substrate linked assay. At a PAF - AH concentration of 10.0 ug/ml, 50% cleavage was achieved at an incubation time of approximately 2 minutes.Storage: The lyophilized PAF-AH recombinant protein is stable for at least 2 years from date of receipt at -20C. Reconstituted PAF-AH is stable for at least 3 months when stored in working aliquots with a carrier protein at -20C. As with any protein, exposing PAF-AH recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.