Secreted FRP-1 Protein (OPPB00127)

Secreted Frizzled Related Proteins (sFRPs) modulate WNT signaling by binding directly to WNT proteins in a manner that affects their receptor binding and signaling capabilities. sFRP-1 is a widely distributed protein that can bind directly to WNT1, WNT2, and possibly other WNT proteins, and generally exerts anti-proliferative effects consistent with activity as a WNT antagonist. It also inhibits apoptosis and has been found to be down-regulated in many solid tumors, but up-regulated in uterine leiomyomas. Recombinant human sFRP-1 is a glycosylated 283 amino acid protein containing a cysteine rich Frizzled homologous domain.

Preparation: Biological activity was determined by its ability to inhibit BMP - 2 proliferation effect of ATDC - 5 cells. The expected ED50 is 0.3–0.5 ug/ml.Storage: The lyophilized Secreted FRP-1 recombinant protein is stable for at least 2 years from date of receipt at -20C. Reconstituted Secreted FRP-1 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20C. As with any protein, exposing Secreted FRP-1 recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.