Recombinant FGF-basic 154 (OPSD00077)

Hersteller AVIVA Systems Biology
Typ Proteins
Specific against other
Menge 10 ug
ArtNr OPSD00077
eClass 6.1 34160400
eClass 9.0 42020190
Molecular weight
Monomer, 17.3 kDa (155 aa)
Product format
Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, 75 mM sodium chloride, pH 7.5
Reconstitution and storage
Prior to reconstitution, store at -20C. Reconstitute with sterile water at 0.1 mg/ml. Centrifuge vial before opening. Suspend the product by gently pipetting sterile water down the down the sides of the vial. Do not vortex. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80C and avoid repeat freeze thaws. Upon reconstitution, a small amount of visible precipitate can be expected. A 10% overfill has been added to the total material vialed to compensate for this loss.
Expiration Date: 12 months from date of receipt when stored at -20C to -80C as supplied.
1 month when stored at 4C after reconstituting as directed.
3 months when stored at -20C to -80C after reconstituting as directed.
Description of target
Basic fibroblast growth factor (FGF-basic), also known as FGF-2, is expressed by endothelial cells and is a mediator of angiogenesis. FGF-basic also has cardioprotective functions during heart injury. FGF-basic is a critical component for embryonic stem cell culture systems and is necessary for maintaining cells in an undifferentiated state. Recombinant FGF-basic 154 is the full length FGF-basic protein encoded by the human FGF-2 gene. FGF-basic 154 is the most popular tissue culture product at Shenandoah Biotechnology, Inc. There are no detectable differences in biological activity between FGF-basic 154 and the truncated FGF-basic 147 proteins.
Biological activity
Determined by NR6R-3T3 Cell Proliferation
Result: ED50= 0.0507 ng/ml (2.0 x 107 units/mg)
> 95% determined by Reducing and Non-reducing SDS-PAGE

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Menge: 10 ug
Lieferbar: In stock


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