Human IL-10 ELISA Kit

This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human IL-10. The Human IL-10 ELISA is for research use only. Not for use in therapeutic procedures. The IL-10 protein is a homodimer, each of its subunits is 178-amino-acid long. In humans, IL-10 is encoded by the IL10 gene, which is located on chromosome 1 and comprises 5 exons, and is primarily produced by monocytes and, to a lesser extent, lymphocytes, namely type 2 T helper cells (Th2), mastocytes, CD4+CD25+Foxp3+ regulatory T cells, and in a certain subset of activated T cells and B cells. IL-10 is a cytokine with multiple, pleiotropic, effects in immunoregulation and inflammation. It downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-kappaB activity, and is involved in the regulation of the JAK-STAT signaling pathway. The immunosuppressive properties of IL-10 suggest a possible clinical use of IL-10 in suppressing rejections of grafts after organ transplantations. IL-10 can furthermore exert strong anti-inflammatory activities.

Sandwich ELISA

serum, plasma, cell culture supernates

4C (unopened)
standard stored at -20C, others stored at 4C (opened)

0.59 pg/ml

81 % - 103 %

n.d. - 35.21 pg/ml

3.2 % - 5.8 %