Human Fas/TNFRSF6/CD95 ELISA Kit

Three samples were tested on one plate

3-4h

Fas, also known as APO-1, or CD95, is a cell-surface receptor that transduces apoptotic signalsfrom Fas ligand (FasL) .Fas forms the death-inducing signaling complex (DISC) upon ligand binding. Membrane-anchored Fas ligand trimer on the surface of an adjacent cell causes oligomerization of Fas. Recent studies which suggested the trimerization of Fas could not be validated. Other models suggested the oligomerization up to 5-7 Fas molecules in the DISC.Although Fas has been shown to promote tumor growth in the above mouse models, analysis of the human cancer genomics database revealed that FAS is not significantly focally amplified across a dataset of 3131 tumors (FAS is not an oncogene), but is significantly focally deleted across the entire dataset of these 3131 tumors, suggesting that FAS functions as a tumor suppressor in humans.In cultured cells, FasL induces various types of cancer cell apoptosis through the Fas receptor. In AOM-DSS-induced colon carcinoma and MCA-induced sarcoma mouse models, it has been shown that Fas acts as a tumor suppressor.Furthermore, the Fas receptor also mediates tumor-specific cytotoxic T lymphocyte (CTL) anti-tumor cytotoxicity. Serum Fas has been reported to be elevated in cancer patients, possibly originating in the tumor cell itself , and in autoimmune diseases.

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solid

0.02% sodium azide

This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of rat VEGF. The Rat VEGF ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Vascular endothelial growth factor (VEGF) is a sub-family of growth factors, to be specific, the platelet-derived growth factor family of cystine-knot growth factors. They are important signaling proteins involved in both vasculogenesis (the denovoformation of the embryonic circulatory system) and angiogenesis (the growth of blood vessels from pre-existing vasculature).VEGF is a signal protein produced by cells that imulates vasculogenesis and angiogenesis. It is part of the system that restores the oxygen supply to tissues when blood circulation is inadequate. VEGF is best known for its role in vasculogenesis. During embryogenesis, VEGF regulates the proliferation, migration, and survival of endothelial cells, thus regulating blood vessel density and size, but playing no role in determining vascular patterns.
Serum concentration of VEGF is high in bronchial asthma and diabetes mellitus. When VEGF is overexpressed, it can contribute to disease. Solid cancers cannot grow beyond a limited size without an adequate blood supply; cancers that can express VEGF are able to grow and metastasize. Overexpression of VEGF can cause vascular disease in the retina of the eye and other parts of the body. Drugs such as bevacizumab and Ranibizumab can inhibit VEGF and control or slow those diseases.

Online product mannual

Online product mannual

100µL

Colorimetric