Anti-Human CD8a BG Violet 450

The OKT8 monoclonal antibody specifically reacts with human CD8α molecule, a tyPEI transmembrane glycoprotein of 32-34 kDa. CD8α is a member of the Ig superfamily, expressed as a homodimer (CD8αα) or as a heterodimer (CD8αβ). CD8+ αβ T lymphocytes express both CD8αα and CD8αβ, while some T lymphocytes and the natural killer cells express only the homodimers. CD8 binds to MHC class I and influences the development and the activation of T lymphocytes.

OKT8, RPA-T8, and HIT8a antibodies do not compete with each other for binding to peripheral leukocytes, meaning that that they do not recognize the same epitoPEor block each other by steric hindrance.

BG Violet 450 conjugate is an alternative to the Pacific Blue, eFluor 450, or BD Horizon V450 dyes. It is excited by the violet (405 nm) laser, with a peak emission of 450nm.

Determined by its ability to chemoattract IL-2 activated T cells using a concentration range of 0.1-10.0 ng/ml.
Assay #2: Determined by its ability to chemoattract CXCR3 transfected/HEK293 cells using a con

Immunology, Angiogenesis, Neurosciences, Inflammation, Proliferation and Cell Cycle, Chemotaxis