Dog Immunoglobulin G (IgG) ELISA Kit (CLIA)

Hersteller Biomatik
Typ Clia
Specific against Canine
Format 96 well plate
Applikationen CLIA
Menge 96T
ArtNr BM-EKU08627
Voraussichtliche Lieferzeit 2-3 Weeks
eClass 6.1 27150207
eClass 9.0 27150207
Detection Range
1.17-300 ug/mL
0.52 ug/mL
Sample Type
Serum, plasma and other biological fluids
Assay Time
2 hours
Gene Name
Immunoglobulin G
Assay Type
Detection Method
Shipping Condition
Blue ice
Storage Instructions
-20C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
Precaution of Use
Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
Expiry Date
8 months
This assay has high sensitivity and excellent specificity for detection of Immunoglobulin G (IgG). No significant cross-reactivity or interference between Immunoglobulin G (IgG) and analogues was observed.
Test Principle
The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Immunoglobulin G (IgG). A competitive inhibition reaction is launched between biotin labeled Immunoglobulin G (IgG) and unlabeled Immunoglobulin G (IgG) (Standards or samples) with the pre-coated antibody specific to Immunoglobulin G (IgG). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Immunoglobulin G (IgG) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Immunoglobulin G (IgG) level in the sample or standard.
Assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Immunoglobulin G (IgG) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Immunoglobulin G (IgG) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
For research use only. Not for diagnostic procedures.
Quality Systems
The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
Research Area
Infection immunity; Immune molecule; Hematology;

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Menge: 96T
Lieferbar: In stock


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